

@article {14553,
	title = {Environmental surveillance system to track wild poliovirus transmission},
	journal = {Appl Environ Microbiol},
	volume = {69},
	year = {2003},
	month = {may},
	pages = {2919{\textendash}27},
	abstract = {Eradication of poliomyelitis from large metropolis cities in India has been difficult due to high population density and the presence of large urban slums. Three paralytic poliomyelitis cases were reported in Mumbai, India, in 1999 and 2000 in spite of high immunization coverage and good-quality supplementary immunization activities. We therefore established a systematic environmental surveillance study by weekly screening of sewage samples from three high-risk slum areas to detect the silent transmission of wild poliovirus. In 2001, from among the 137 sewage samples tested, wild poliovirus type 1 was isolated from 35 and wild poliovirus type 3 was isolated from 1. Acute flaccid paralysis (AFP) surveillance indicated one case of paralytic poliomyelitis from the city. Phylogenetic analysis with complete VP1 sequences revealed that the isolates from environmental samples belonged to four lineages of wild polioviruses recently isolated from poliomyelitis cases in Uttar Pradesh and not to those previously isolated from AFP cases in Mumbai. Wild poliovirus thus introduced caused one case of paralytic poliomyelitis. The virus was detected in environmental samples 3 months before. It was found that wild polioviruses introduced several times during the year circulated in Mumbai for a limited period before being eliminated. Environmental surveillance was found to be sensitive for the detection of wild poliovirus silent transmission. Nucleotide sequence analysis helped identify wild poliovirus reservoir areas.},
	keywords = {Base Sequence, Capsid Proteins/genetics, Environmental Microbiology, Genome, Humans, India, Molecular Sequence Data, Oral/genetics, Poliomyelitis/prevention \& control/*transmission, Poliovirus Vaccine, Poliovirus/genetics/*isolation \& purification, Population Surveillance/*methods, Poverty Areas, RNA, Sensitivity and Specificity, Sewage/virology, Urban Health, Viral, Viral/genetics},
	issn = {0099-2240 (Print) 0099-2240 (Linking)},
	author = {Deshpande, J. M. and Shetty, S. J. and Siddiqui, Z. A.}
}
