

@article {14121,
	title = {Abundance and distribution of bacteria carrying {sltII} gene in natural river water},
	journal = {Lett Appl Microbiol},
	volume = {28},
	number = {5},
	year = {1999},
	pages = {405{\textendash}410},
	abstract = {Direct in situ PCR with HNPP/Fast Red TR was used to enumerate bacteria carrying the sltII gene in river water. By direct in situ PCR with a sltII-specific EVS primer, 10(2)-10(5) cells ml-1 of bacteria carrying the sltII gene were detected from all sampling sites, except the site nearest to the source of the river, while 10(2)-10(4) cells ml-1 of Escherichia coli O157:H7 were detected using a direct fluorescent antibody staining method. These results indicate that such bacteria are commonly distributed in natural river water. Direct in situ PCR with HNPP/Fast Red TR is a useful tool for detecting cells carrying specific genes, such as verotoxin-producing bacteria in natural environments.},
	author = {Kurokawa, K and Tani, K and Ogawa, M and Nasu, M}
}
