

@article {13901,
	title = {Prevalence and potential link between {E}. coli {O}157:{H}7 isolated from drinking water, meat and vegetables and stools of diarrhoeic confirmed and non-confirmed {HIV}/{AIDS} patients in the {Amathole} {District} - {South} {Africa}.},
	journal = {Journal of applied microbiology},
	volume = {105},
	number = {2},
	year = {2008},
	month = {aug},
	pages = {424{\textendash}31},
	abstract = {AIM: The current study investigated the prevalence and molecular relatedness between Escherichia coli O157:H7 isolated from water, meat and meat products and vegetables and from stools of confirmed and non-confirmed Human Immune Virus/Acquired Immunodeficiency Syndrome (HIV/AIDS) patients with diarrhoea. METHODS AND RESULTS: Culture-based and polymerase chain reaction techniques were used to identify E. coli O157:H7. Thirty-five per cent of meat products, 25.5\% of water, 21.7\% of vegetables as well as 56.5\% and 43.5\% of stools of confirmed and non-confirmed HIV/AIDS patients, respectively, were presumptively positive with E. coli O157. Molecular results indicated that 10.3\%, 8.6\% and 7.8\% of the vegetables, water and meat products examined carried E. coli O157:H7, which had homologous fliC(H7), rfbE(O157) and eaeA genetic loci to the genes of some E. coli O157:H7 isolated from 12.2\% and 8.8\% of the stools of confirmed and non-confirmed HIV/AIDS patients, respectively. CONCLUSIONS: Water, meat and meat products and vegetables are potential sources of E. coli O157:H7 that are potentially capable of causing diarrhoea in humans especially HIV/AIDS patients. SIGNIFICANCE AND IMPACT OF THE STUDY: Great care should be exercised to ensure that water and foods consumed by HIV/AIDS patients are safe, as contaminated water and foods can cause secondary infections in these patients.},
	issn = {1365-2672},
	doi = {10.1111/j.1365-2672.2008.03756.x},
	author = {Abong{\textquoteright}o, B O and M NB Momba}
}
