

@article {13767,
	title = {Development of a virus concentration method and its application to detection of enterovirus and {Norwalk} virus from coastal seawater},
	journal = {Applied and Environmental Microbiology},
	volume = {68},
	number = {3},
	year = {2002},
	abstract = {We developed a new procedure for concentration of enteric viruses from water using a negatively charged membrane. Rinsing the membrane with 0.5 mM H2SO4 (pH 3.0) in order to elute cations prior to viral elution with 1 mM NaOH (pH 10.5) promoted poliovirus recovery yields from 33 to 95\% when applied to pure water and 38 to 89\% when applied to natural seawater from Tokyo Bay, Japan, respectively. This method showed average recovery yields of spiked poliovirus of 62\% (n = 8) from 1 liter of artificial seawater. This method showed higher recovery yields ({\textbackslash}textgreater61\%) than that of the conventional method using positively charged membrane (6\%) when applied to seawater. This method is also free from beef extract elution, which has an inhibitory effect in the subsequent viral genome detection by reverse transcription-PCR. Naturally occurring Norwalk viruses from 2 liters of Tokyo Bay water in winter and infectious enteroviruses from 2 liters of recreational coastal seawater in summer were detected by using this viral concentration method.},
	doi = {10.1128/AEM.68.3.1033-1039.2002},
	author = {Katayama, H. and Shimasaki, A. and Ohgaki, S.}
}
