

@article {1267,
	title = {Survival of adenovirus types 2 and 41 in surface and ground waters measured by a plaque assay},
	journal = {Environ Sci Technol},
	volume = {45},
	number = {9},
	year = {2011},
	note = {Rigotto, C Hanley, K Rochelle, P A De Leon, R Barardi, C R M Yates, M V Research Support, Non-U.S. Gov{\textquoteright}t United States Environmental science \& technology Environ Sci Technol. 2011 May 1;45(9):4145-50. doi: 10.1021/es103922r. Epub 2011 Apr 11.},
	pages = {4145-50},
	type = {Journal Article},
	abstract = {To manage artificial recharge systems, it is necessary to understand the inactivation process of microorganisms within aquifers so that requirements regarding storage times and treatment strategies for ground and surface waters can be developed and modeled to improve water management practices. This study was designed to investigate the survival of representative adenoviruses in surface- and groundwaters using a cell culture plaque assay with human lung carcinoma cells (A549) to enumerate surviving viruses. Adenovirus types 2 (Ad2) and 41 (Ad41) were seeded into 50 mL of three sterilized surface waters and groundwaters, and incubated at 10 and 19 degrees C for up to 301 days. Concentrations of Ad2 and Ad41 were relatively stable in all waters at 10 degrees C for at least 160 days and in some instances up to 301 days. At 19 degrees C, virus concentrations were reduced by 99.99\% (4 log) after 301 days in surface water. There was approximately 90\% (1 log) reduction of both viruses at 19 degrees C after 160 days of incubation in groundwater samples. There was no overall difference in survival kinetics in surface waters compared to groundwaters. The relatively high stability and long-term survival of adenoviruses in environmental waters at elevated temperatures should be considered in risk assessment models and drinking water management strategies.},
	keywords = {Adenoviridae/ physiology Adenoviridae Infections/virology Cell Line, Tumor Fresh Water/ virology Humans Temperature Viral Plaque Assay Water Microbiology Water Supply},
	issn = {1520-5851 (Electronic) 0013-936X (Linking)},
	doi = {10.1021/es103922r},
	author = {Rigotto, C. and Hanley, K. and Rochelle, P. A. and De Leon, R. and Barardi, C. R. and Yates, M. V.}
}
