

@article {12553,
	title = {Shift in antibiotic resistance gene profiles associated with nanosilver during wastewater treatment},
	journal = {Fems Microbiology Ecology},
	volume = {92},
	number = {3},
	note = {Ma, Yanjun Metch, Jacob W. Yang, Ying Pruden, Amy Zhang, Tong<br/>Zhang, Tong/C-6786-2008<br/>1574-6941},
	month = {Mar},
	abstract = {This study investigated the response of antibiotic resistance genes (ARGs) to nanosilver (Ag) in lab-scale nitrifying sequencing batch reactors (SBRs), compared to Ag+-dosed and undosed controls. Quantitative polymerase chain reaction (q-PCR) targeting sul1, tet(O), ermB and the class I integron gene intI1 and corresponding RNA expression did not indicate measureable effects of nanoAg or Ag+ on abundance or expression of these genes. However, high-throughput sequencing based metagenomic analysis provided a much broader profile of gene responses and revealed a greater abundance of aminoglycoside resistance genes (mainly strA) in reactors dosed with nanoAg. In contrast, bacitracin and macrolide-lincosamide-streptogramin (MLS) resistance genes were more abundant in the SBRs dosed with Ag+. The distinct ARG profiles associated with nanoAg and Ag+ were correlated with the taxonomic composition of the microbial communities. This study indicates that nanoAg may interact with bacteria differently from Ag+ during biological wastewater treatment. Therefore, it cannot necessarily be assumed that nanosilver behaves identically as Ag+ when conducting a risk assessment for release into the environment.},
	isbn = {0168-6496},
	author = {Ma, Y. J. and Metch, J. W. and Yang, Y. and Pruden, A. and Zhang, T.}
}
