

@article {12326,
	title = {Metagenomic Profiling of Microbial Composition and Antibiotic Resistance Determinants in Puget Sound},
	journal = {PLoS One},
	number = {10},
	year = {2012},
	note = {Port, Jesse A. Wallace, James C. Griffith, William C. Faustman, Elaine M.<br/>Faustman, Elaine/0000-0002-3085-6403},
	month = {Oct},
	abstract = {<p>Human-health relevant impacts on marine ecosystems are increasing on both spatial and temporal scales. Traditional indicators for environmental health monitoring and microbial risk assessment have relied primarily on single species analyses and have provided only limited spatial and temporal information. More high-throughput, broad-scale approaches to evaluate these impacts are therefore needed to provide a platform for informing public health. This study uses shotgun metagenomics to survey the taxonomic composition and antibiotic resistance determinant content of surface water bacterial communities in the Puget Sound estuary. Metagenomic DNA was collected at six sites in Puget Sound in addition to one wastewater treatment plant (WWTP) that discharges into the Sound and pyrosequenced. A total of similar to 550 Mbp (1.4 million reads) were obtained, 22 Mbp of which could be assembled into contigs. While the taxonomic and resistance determinant profiles across the open Sound samples were similar, unique signatures were identified when comparing these profiles across the open Sound, a nearshore marina and WWTP effluent. The open Sound was dominated by alpha-Proteobacteria (in particular Rhodobacterales sp.), gamma-Proteobacteria and Bacteroidetes while the marina and effluent had increased abundances of Actinobacteria, beta-Proteobacteria and Firmicutes. There was a significant increase in the antibiotic resistance gene signal from the open Sound to marina to WWTP effluent, suggestive of a potential link to human impacts. Mobile genetic elements associated with environmental and pathogenic bacteria were also differentially abundant across the samples. This study is the first comparative metagenomic survey of Puget Sound and provides baseline data for further assessments of community composition and antibiotic resistance determinants in the environment using next generation sequencing technologies. In addition, these genomic signals of potential human impact can be used to guide initial public health monitoring as well as more targeted and functionally-based investigations.</p>
},
	isbn = {1932-6203},
	author = {Port, J. A. and Wallace, J. C. and Griffith, W. C. and Faustman, E. M.}
}
