@article {12263, title = {Experimental Design and Bioinformatics Analysis for the Application of Metagenomics in Environmental Sciences and Biotechnology}, journal = {Environmental Science and Technology}, volume = {49}, number = {21}, year = {2015}, note = {Ju, Feng Zhang, Tong
Zhang, Tong/C-6786-2008; JU, Feng/I-8437-2016
1520-5851}, month = {Nov}, pages = {12628-12640}, abstract = {

Recent advances in DNA sequencing technologies have prompted the widespread application of metagenomics for the investigation of novel bioresources (e.g., industrial enzymes and bioactive molecules) and unknown biohazards (e.g., pathogens and antibiotic resistance genes) in natural and engineered microbial systems across multiple disciplines. This review discusses the rigorous experimental design and sample preparation in the context of applying metagenomics in environmental sciences and biotechnology. Moreover, this review summarizes the principles, methodologies, and state-of-the-art bioinformatics procedures, tools and database resources for metagenomics applications and discusses two popular strategies (analysis of unassembled reads versus assembled contigs/draft genomes) for quantitative or qualitative insights of microbial community structure and functions. Overall, this review aims to facilitate more extensive application of metagenomics in the investigation of uncultures microorganisms, novel enzymes, microbe-environment interactions, and biohazards in biotechnological applications microbial communities are engineered for bioenergy production, wastewater treatment, and bioremediation.

}, isbn = {0013-936X}, author = {Ju, F. and Zhang, T.} }