

@article {11758,
	title = {{Two-dimensional immunoblot analysis of antigenic proteins of Spirometra plerocercoid recognized by human patient sera.}},
	journal = {International Journal for Parasitology},
	volume = {60},
	number = {2},
	year = {2011},
	pages = {139{\textendash}143},
	abstract = {<p>Sparganosis is caused by invasion of Spirometra plerocercoid into various tissues/organs. Subcutaneous sparganosis can be diagnosed and treated by worm removal, while visceral/cerebral sparganosis is not easy to diagnose. The diagnosis depends largely on the detection of specific antibodies circulating in the patients\&$\#$39; sera. Previous studies demonstrated that 31 and {\{}36kDa{\}} proteins of the sparganum invoked specific and sensitive antibody responses, but also showed cross reactions with cysticercosis sera. We enriched protein fractions containing {\{}31-36kDa{\}} through gel filtration and examined immune recognition pattern against the patient sera by 2-dimensional electrophoresis {\{}(2-DE){\}} followed by immunoblotting. Serum samples from sparganosis patients recognized 8-10 protein spots of 31 and {\{}36kDa{\}} with different isoelectric point {\{}(pI){\}} values with variable combinations, in which four spots of {\{}31kDa{\}} with {\{}pIs{\}} 3.4, 3.9, 4.0 and 4.1, and one {\{}36kDa{\}} spot {\{}(pI{\}} 3.5) appeared to be specifically reactive. One {\{}31kDa{\}} protein spot with {\{}pI{\}} 3.3 and two spots of {\{}36kDa{\}} with {\{}pIs{\}} 3.3 and 3.5 reacted crossly with neurocysticercosis sera. Neither sera from patients with other parasitic infections nor those from healthy controls showed positive reaction. {\{}Two-DE/immunoblot{\}} analysis might be highly available in differential serodiagnosis of human sparganosis.</p>
},
	issn = {1383-5769},
	doi = {10.1016/j.parint.2011.01.002},
	author = {Rahman, Moizur and Lee, Eung-Goo and Bae, Young-An}
}
