

@article {10966,
	title = {A {Method} for {Assessing} the {Viability} of {Nematode} {Eggs} in {Sludge}},
	journal = {Environmental Technology},
	volume = {17},
	number = {4},
	year = {1996},
	month = {apr},
	pages = {415{\textendash}420},
	abstract = {Helminth eggs are found in urban sludge used in agriculture. Then, to evaluate the parasitic risk, a method was developed to estimate the viability of these eggs. The extraction of these eggs was performed with a diphasic treatment (SDS 0.01 \% - Ethylacetate) coupled with a filtration on 500 {\textmu}m and 100 {\textmu}m sieves followed by concentration using two flotations with NaCl (d = 1.19). For the culture, 3 parameters tested showed a faster egg development at 30{\textdegree}C in deionized water with continuous aeration, whereas organic compounds reduced this development. This culture was performed during respectively 13, 10 and 8 days to obtain Ascaris suum, Toxocara canis and Capillaria sp larva, and 16 days for Trichuris vulpis which presented a slower development. The quantification was realized by microscopic examination (100 X) after sonication and sodium hypochlorite (0.5{\textdegree} Chl) treatment. The eggs reaching the larva stage were considered as viable and the percent of viability was expressed by the ratio: nematode eggs at the larva stage/total of Nematode eggs counted.},
	issn = {0959-3330},
	doi = {10.1080/09593331708616401},
	url = {https://doi.org/10.1080/09593331708616401},
	author = {Gaspard, Philippe and Wiart, Jacques and Schwartzbrod, Janine}
}
